INFLUÊNCIA DE PRÉ-TRATAMENTOS NA PRODUÇÃO DE CAROTENOIDES EM MEIO AGROINDUSTRIAL POR SPORODIOBOLUS PARAROSEUS
Resumo
Os carotenoides podem ser obtidos por processos químicos ou biologicamente por micro-organismos, com destaque para Sporidiobolus pararoseus, apresentando crescente interesse nesta via de obtenção em função da grande aplicabilidade desses biocompostos. Esta levedura é promissora para cultivos submersos, apresentando fácil assimilação de coprodutos agroindustriais, fontes alternativas de carbono e nitrogênio, usados como substratos nos meios de produção. Neste estudo, avaliou-se o uso de um pré-tratamento químico (ácido sulfúrico ou fosfórico) ou físico (carvão ativo) nos substratos alternativos para a bioprodução de carotenoides, usando a S. pararoseus. Nas condições de processo a 25 ºC, 180 rpm em 168 h com o meio de produção agroindustrial 40 g/L de melaço de cana de açúcar e 6,5 g/L de água de maceração de milho pré-tratado com ácido sulfúrico foi possível alcançar 936,35 µg/L (79.63 µg/g) de carotenoides totais, 12,02 g/L de biomassa e produtividade em carotenoide (Pc) de 5,57 µg/L.h (0.47 µg/g.h). A bioprodução de carotenoides alcançou 812,32 µg/L (72,35 µg/g), 11,27 g/L de biomassa e Pc de 4,83 µg/L.h (0,43 µg/g.h) para ácido fosfórico. Portanto, os pré-tratamentos com ácido sulfúrico e ácido fosfórico nos substratos agroindustriais foram promissores e promoveram incrementos superiores a 55% e 79% na concentração de carotenoides totais, respectivamente.
Referências
AI, H.; LIU, M.; YU, P.; ZHANG, S.; SUO, Y.; LUO, P.; LI, S. & WANG, J. Improved welan gum production by Alcaligenes sp. ATCC31555 from pretreated cane molasses. Carbohydrate Polymers Vol. 129, p.35–43, 2015.
AOAC. Official Methods of Analysis of AOAC International. 19th ed. USA: Association of Official Analysis Chemists International., 2012.
ARSAD, M.; HUSSAIN, T.; IQBAL, M. & ABBAS, M. Enhanced ethanol production at commercial scale from molasses using high gravity technology by mutant S. cerevisiae. Brazilian Journal of Microbiology Vol. 48, n.3, p.403–409, 2017.
BERWANGER, A.L.S.; DOMINGUES, N.M.; VANZO, L.T.; LUCCIO, M.; TREICHEL, H.; PADILHA, F.F. & SCAMPARINI, A.R.P. Production and rheological characterization of biopolymer of Sphingomonas capsulata ATCC 14666 using conventional and industrial media. Applied biochemistry and biotechnology Vol. 129–132, p.942–50, 2006.
CHENG, Y.T. & YANG, C.F. Using strain Rhodotorula mucilaginosa to produce carotenoids using food wastes. Journal of the Taiwan Institute of Chemical Engineers Vol. 61, p.270–275, 2016.
CIPOLATTI, E.; BULSING, B.; SÁ, C.S.; BURKERT, C.A.V.; FURLONG, E.B. & BURKERT, J.F.M. Carotenoids from Phaffia rhodozyma: Antioxidant activity and stability of extracts. African Journal of Biotechnology Vol. 14, n.23, p.1982–1988, 2015.
COLET, R.; URNAU, L.; BAMPI, J.; ZENI, J.; DIAS, B.B.; RODRIGUES, E.; JACQUES, R.A.; LUCCIA, M.D. & VALDUGA, E. Use of low-cost agro products as substrate in semi-continuous process to obtain carotenoids by Sporidiobolus salmonicolor. Biocatalysis and Agricultural Biotechnology Vol. 11, p.268–274, 2017.
DAVIES, B.H. Chemistry and Biochemistry of Plant Pigments. In: GOODWIN, T. (Ed.). Carotenoids. London: Academic Press, p.38–165, 1976.
DESHMUKH, A.N.; GOKHALE, P.N. & JAIN, R. Engineering of Bacillus subtilis for the Production of 2,3-Butanediol from Sugarcane Molasses. Applied Biochemistry and Biotechnology Vol. 179, n.2, p.321–331, 2016.
EZEJIOFOR, T.; ENEBAKU, U.E. & OGUEKE, C. Waste to Wealth-Value Recovery from Agro-food Processing Wastes Using Biotechnology: A Review. British Biotechnology Journal Vol. 4, n.4, p.418–481, 2014.
FARIAS, F.O.; ALBERTI, A.; NOGUEIRA, A. & DEMIATE, I.M. Mixture design applied to the study of bioethanol production from cheese whey and corn steep liquor. Brazilian Journal of Food Research Vol. 7, n.3, p.150, 2016.
FEKETEA, G. & TSABOURI, S. Common food colorants and allergic reactions in children: Myth or reality?. Food Chemistry Vol. 230, p.578–588, 2017.
FILLET, S.; GIBERT, J.; SUÁREZ, B.; LARA, A.; RONCHEL, C. & ADRIO, J.L. Fatty alcohols production by oleaginous yeast. Journal of Industrial Microbiology & Biotechnology, Vol. 42, n.11, p.1463–1472, 2015.
FONSECA, R. & RAFAEL, R.D.S. Different cell disruption methods for astaxanthin recovery by Phaffia rhodozyma. African Journal of Biotechnology, Vol. 10, n.7, p.1165–1171, 2011.
FRENGOVA, G.; SIMOVA, E.; PAVLOVA, K. & GRIGROVA, D. Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate. Biotechnology and bioengineering Vol. 44, n.8, p.888–94, 1994.
GAO, Y. & YUAN, Y.J. Comprehensive quality evaluation of corn steep liquor in 2-keto-L-gulonic acid fermentation. Journal of Agricultural and Food Chemistry Vol. 59, n.18, p.9845–9853, 2011.
HAN, M.; DU, C.; XU, Z.Y.; QIAN, E. & ZHANG, W.G. Rheological properties of phosphorylated exopolysaccharide produced by Sporidiobolus pararoseus JD-2. International Journal of Biological Macromolecules Vol. 88, p.603–613, 2016.
HOU, J. & CUI, H.L. In vitro antioxidant, antihemolytic, and anticancer activity of the carotenoids from Halophilic archaea. Current Microbiology Vol. 75, n.3, p.266–271, 2018.
KAUR, A.; GUPTA, V.; CHRISTOPHER, A.F.; MALIK, M.A. & BANSAL, P. Nutraceuticals in prevention of cataract – An evidence based approach. Saudi Journal of Ophthalmology Vol. 31, n.1, p.30–37, 2017.
KAYAHAN, E.; EROGLU, I. & KOKU, H.A. Compact tubular photobioreactor for outdoor hydrogen production from molasses. International Journal of Hydrogen Energy Vol. 42, n.4, p.2575–2582, 2017.
KONOPACKA, D.; CYBULSKA, J.; ZDUNEK, A.; DYKI, B.; MACHLAŃSKA, A. & CELEJEWSKA, K. The combined effect of ultrasound and enzymatic treatment on the nanostructure, carotenoid retention and sensory properties of ready-to-eat carrot chips. LWT - Food Science and Technology Vol. 85, p.427–433, 2017.
KULCZYŃSKI, B.; MICHALOWSKA, A.G.; CISOWSKA, J.K. & KMIECIK, D. The role of carotenoids in the prevention and treatment of cardiovascular disease – Current state of knowledge. Journal of Functional Foods Vol. 38, p.45–65, 2017.
KUSDIYANTINI, E.; GAUDIN, P.; GOMA, G. & PHILIPPE, J.B. Growth kinetics and astaxanthin production of Phaffia rhodozyma on glycerol as a carbon source during batch fermentation. Biotechnology Letters Vol. 20, n.10, p.929–934, 1998.
LOPES, N.A.; REMEDI, R.D.; SANTOS, S.C.; BURKERT, C.A.V. & BURKERT, J.F.M. Different cell disruption methods for obtaining carotenoids by Sporodiobolus pararoseus and Rhodothorula mucilaginosa. Food Science and Biotechnology Vol. 26, n.3, p.759–766, 2017.
MACHADO, W. R. C. & BURKERT, J. F. M. Optimization of agroindustrial medium for the production of carotenoids by wild yeast Sporidiobolus pararoseus. African Journal of Microbiology Research Vol. 9, n.4, p.209–219, 2015.
MANOWTTANA, A.; TECHAPUN, C.; WATANBE, M. & CHAIYASO, T. Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift bioreactor. Journal of Bioscience and Bioengineering, Vol. 125, n.1, p.59–66, 2018.
MARKETS e MARKETS. Carotenoids Market by Type (Astaxanthin, Beta-Carotene, Canthaxanthin, Lutein, Lycopene, & Zeaxanthin), Source (Synthetic and Natural), Application (Supplements, Food, Feed, and Cosmetics), & by Region - Global Trends & Forecasts to 2021. Disponível em: <https://www.marketsandmarkets.com/Market-Reports/carotenoid-market-158421566.html>. Acesso em: 24 out. 2018.
MICHELON, M.; MATOS, B.T.; SILVA, R.R.; BURKERT, C.A.V. & BURKERT, J.F.M. Extraction of carotenoids from Phaffia rhodozyma: A comparison between different techniques of cell disruption. Food Science and Biotechnology Vol. 21, n.1, p.1–8, 2012.
MISCHOPOULOU, M. NAIDIS, P.; KALAMARAS, S.; KOTSOPOULOS, T.A. & SAMARAS, P. Effect of ultrasonic and ozonation pretreatment on methane production potential of raw molasses wastewater. Renewable Energy Vol. 96, p.1078–1085, 2016.
NASCIMENTO, W.C.A.; SILVA, C.R.; CARVALHO, R.V. & MARTINS, M.L.L. Optmization of a culture medium for protease production by Bacillus sp. thermophilic. Food Science and Technology Vol. 27, n.2, p.417–421, 2007.
NIU, F.X.; LU, Q.; BU, Y.F. & LIU, J.Z. Metabolic engineering for the microbial production of isoprenoids: Carotenoids and isoprenoid-based biofuels. Synthetic and Systems Biotechnology Vol. 2, n.3, p.167–175, 2017.
PAN, N.C., PEREIRA, H.C.B., SILVA, M.L.C.; VASCONCELOS, A.F.D.; & CELLIGOI, M.A.P.C. Improvement production of hyaluronic acid by Streptococcus zooepidemicus in sugarcane molasses. Applied Biochemistry and Biotechnology Vol. 182, n.1, p.276–293, 2017.
PARAJÓ, J.C.; SANTOS, V. & VÁZQUEZ, M. Optimization of carotenoid production by Phaffia rhodozyma cells grown on xylose. Process Biochemistry Vol. 33, n.2, p.181–187, 1998.
RIOS, D.A.S.; BORBA, T.M.; KALIL, S.J. & BURKERT, J.F.M. Rice parboiling wastewater in the maximization of carotenoids bioproduction by Phaffia rhodozyma. Ciência e Agrotecnologia Vol. 39, n.4, p.401–410, 2015.
ROOHBAKHSH, A.; KARIMI, G. & IRANSHAHI, M. Carotenoids in the treatment of diabetes mellitus and its complications: A mechanistic review. Biomedicine & Pharmacotherapy, Vol. 91, p.31–42, 2017.
ROUKAS, T. Pretreatment of beet molasses to increase pullulan production. Process Biochemistry Vol. 33, n.8, p. 805–810, 1998.
SARIOL, H.C.; YPERMAN, J.; SAUVANELL, A.B.; CARLEER, R.; CAMPA, J.N. & GRYGLEWICZ, G. A novel acoustic approach for the characterization of granular activated carbons used in the rum production. Ultrasonics Vol. 70, p.53–63, 2016.
SGUAREZI, C.M.; LONGO, C.; CENI, G.; BONI, G.; SILVA, M.F.; LUCCIO, M.; MAZUTTI, M.A.; MAUGERI, F.; RODRIGUES, M.I. & TREICHEL, H. Inulinase production by agro-industrial residues: optimization of pretreatment of substrates and production medium. Food and Bioprocess Technology Vol. 2, n.4, p.409–414, 2009.
SILVA, C.M.; BORBA, T.M.; KALIL, S.J. & BURKERT, J.F.M. Raw glycerol and parboiled rice effluent for carotenoid production: Effect of the composition of culture medium and initial pH. Food Technology and Biotechnology Vol. 54, n.4, p.489–496, 2016.
TASKIN, M.; ORTUCU, S.; AYDOGAN, M.N. & ARSLAND, N.P. Lipid production from sugar beet molasses under non-aseptic culture conditions using the oleaginous yeast Rhodotorula glutinis TR29. Renewable Energy Vol. 99, p.198–204, 2016.
VALDUGA, E.; VALÉRIO, A.; TREICHEL, H. & LUCCIO, M. Pré-tratamentos de melaço de cana-de-açúcar e água de maceração de milho para a bioprodução de carotenóides. Química Nova Vol. 30, n.8, p.1860–1866, 2007.
VALDUGA, E.; RIBEIRO, A. H., R.; CÊNCIA, K.; COLET, R.; TINGGEMANN, L.; ZENI, J. &TONIAZZO, G. Carotenoids production from a newly isolated Sporidiobolus pararoseus strain using agroindustrial substrates. Biocatalysis and Agricultural Biotechnology Vol. 3, n.2, p.207–213, 2014.
VECINO, X.L.; PEREIRA, L.B; REY, R.D.; CRUZ, J.M. &MOLDES, A.B. Optimization of liquid–liquid extraction of biosurfactants from corn steep liquor. Bioprocess and Biosystems Engineering Vol. 38, n.9, p.1629–1637, 2015.
WANG, D.; JU, X.; ZHOU, D. & WEI, G. Efficient production of pullulan using rice hull hydrolysate by adaptive laboratory evolution of Aureobasidium pullulans. Bioresource Technology Vol. 164, p.12–19, 2014a.
WANG, F.; HU, J.H.; GUO, C. & LIU, C.Z. Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer. Bioresource Technology Vol. 166, p.602–605, 2014b.
XIA, W.; CHEN, W.; PENG, W. F. & LI, K. T. Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates. Bioprocess and Biosystems Engineering Vol. 38, n.6, p.1065–1073, 2015.
YOO, A.Y.; ALNAEELI, M. & PARK, J.K. Production control and characterization of antibacterial carotenoids from the yeast Rhodotorula mucilaginosa AY-01. Process Biochemistry Vol. 51, n.4, p.463–473, 2016.
YU, X.J.; YU, Z.Q.; LIU, Y. L.; SUN, J.; Zheng, J.Y. & WANG, Z. Utilization of high-fructose corn syrup for biomass production containing high levels of docosahexaenoic acid by a newly isolated Aurantiochytrium sp. YLH70. Applied Biochemistry and Biotechnology Vol. 177, n.6, p.1229–1240, 2015.
ZHENG, H.; MA, X.; GAO, Z.; WAN, Y.; MIN, M.; ZHOU, W.; LI, Y.; LIU, Y. HUANG, H.; CHEN, P. &RUAN, R. Lipid production of heterotrophic Chlorella sp. from hydrolysate mixtures of lipid-extracted microalgal biomass residues and molasses. Applied Biochemistry and Biotechnology Vol. 177, n.3, p.662–674, 2015.
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