INFLUÊNCIA DE PRÉ-TRATAMENTOS NA PRODUÇÃO DE CAROTENOIDES EM MEIO AGROINDUSTRIAL POR SPORODIOBOLUS PARAROSEUS
Abstract
Os carotenoides podem ser obtidos por processos químicos ou biologicamente por micro-organismos, com destaque para Sporidiobolus pararoseus, apresentando crescente interesse nesta via de obtenção em função da grande aplicabilidade desses biocompostos. Esta levedura é promissora para cultivos submersos, apresentando fácil assimilação de coprodutos agroindustriais, fontes alternativas de carbono e nitrogênio, usados como substratos nos meios de produção. Neste estudo, avaliou-se o uso de um pré-tratamento químico (ácido sulfúrico ou fosfórico) ou físico (carvão ativo) nos substratos alternativos para a bioprodução de carotenoides, usando a S. pararoseus. Nas condições de processo a 25 ºC, 180 rpm em 168 h com o meio de produção agroindustrial 40 g/L de melaço de cana de açúcar e 6,5 g/L de água de maceração de milho pré-tratado com ácido sulfúrico foi possível alcançar 936,35 µg/L (79.63 µg/g) de carotenoides totais, 12,02 g/L de biomassa e produtividade em carotenoide (Pc) de 5,57 µg/L.h (0.47 µg/g.h). A bioprodução de carotenoides alcançou 812,32 µg/L (72,35 µg/g), 11,27 g/L de biomassa e Pc de 4,83 µg/L.h (0,43 µg/g.h) para ácido fosfórico. Portanto, os pré-tratamentos com ácido sulfúrico e ácido fosfórico nos substratos agroindustriais foram promissores e promoveram incrementos superiores a 55% e 79% na concentração de carotenoides totais, respectivamente.
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